Olympus 150x 1.45 NA TIRF Objective Offers Outstanding Light Transmission from UV to IR
MELVILLE, NY, September 15, 2005 - A new 150x, 1.45 NA oil objective offers excellent light transmission for total internal reflectance fluorescence (TIRF) research microscopy over an unprecedented range of the spectrum from the ultraviolet (UV) to the infrared (IR). The high-NA Olympus U Apochromat 150X TIRF objective enables easy TIRF observation using a standard coverglass and standard immersion oil. Olympus offers the most comprehensive line of TIRF optics for advanced research.
The objective is the only one currently available that offers both high UV transmission down to 330 nm and a very high numerical aperture, making it possible to perform TIRF microscopy and UV applications such as un-caging and/or photo-activation with just one lens. In addition, the objective offers IR transmission well beyond 1000 nm, making it well suited to IR fluorescence imaging and IR laser applications, and opening up the potential for live-cell microscopy and future applications in the growing IR microscopy field.
The high magnification of the objective enhances small specimen structures on the CCD camera, thus overcoming the resolution limit imposed by the CCD’s pixel size, an advantage because it does not add either the light loss or potential for aberrations that additional magnification optics can cause. In addition, due to its small field of view, the optic’s illumination light is concentrated onto a very small area of the specimen, offering added illumination intensity that makes the objective a good choice for single molecule fluorescence applications.
The 150x NA 1.45 objective offers evanescent wave penetration characteristics similar to the existing Olympus 60x NA 1.45 TIRF objective. It has a working distance of 0.10, a field number of 22, and uses a correction collar to compensate for coverglass thickness and temperature.
For more information on the Olympus U Apochromat 150X TIRF objective , contact Olympus America Inc., Two Corporate Center Drive, P.O. Box 9058, Melville, NY 11747-9058; www.olympusamerica.com/microscopes ; Email: firstname.lastname@example.org; or phone 1-800-455-8236.
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