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FluoView 1000 Confocal Microscope

Product: FluoView 1000 Confocal Microscope [ change ]

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Technical Specs
Olympus FluoView FV1000 Specifications
Laser Source Visible Select from the following lasers to be mounted on laser combiner:
Multi-line Argon Laser (457nm, 488nm, 515nm), total 30mW
473nm Diode Laser 15mW
HeNe-Green Laser (543nm), 1mW, or 559nm Laser Diode, 15mW
635nm Diode Laser 20mW
748nm Laser Diode, 13mW Special order
Violet/UV Select from the following:
405nm Laser Diode, 25mW
440nm Laser Diode, 5.3mW
Laser Control AOTF intensity control and shuttering for each laser line in UV and visible light range. Integrated system control for diode lasers. Both controls provide laser turn-off during retrace period and REX mode scanning. REX (region of excitation) mode permits laser intensity and wavelength selection for each designated region of excitation. Integrated shutters are included for all laser lines.
Laser Feedback Monitor Included within the excitation light path of the main scan unit. Provides continuous intensity feedback to laser control. Maintains consistent laser power to the sample during time series experiments.
Scan Unit Standard Configuration 3 internal PMT detectors for fluorescence detection + 1 external PMT detector for transmitted light, 3 laser ports (visible, UV, IR)

Additional optional light paths available, including:
4th Channel PMT detector unit
Fiber port for fluorescence output to fiber optic
SIM Scanner
Scan Method 2 galvano mirrors
Pinhole One common pinhole for all channels.
Pinhole diameter: 50-800m (50-300m for spectral system)
Pinhole continuously adjustable in 0.5m increments.
Scan Modes Normal, Clip, Zoom-In, Bi-Directional Fast Scan, Line, Point

XY Scan
Dimensions: Time, Z, lambda (for spectral system)
Pixels: 64x64 - 4096x4096
Aspect ratio: 1:1, 4:3, Arbitrary ratio
Rotation: 360-degree
Scan Speed (Pixel duration):
Normal Mode: 2s - 5ms, 9 steps, 500-5KHz
Bi-Directional: 1KHz, (512x512, 2x zoom)
2KHz, (256x256, 5x zoom)

Line Scan
Dimension: Time, Z, lambda (for spectral system)
Type: straight line, rotation, free line
Number of lines: 1 - 32,500 Lines
Bi-Directional: 2-4KHz/line
Fast XZ: 2msec/line
Field Number (FN) 18
Zoom 1x - 50x
0.1x increments
Z-drive Motorized focus through internal stepper motor.
Minimum step increment 0.01m.
PMT Detection High-sensitivity, side-on PMT detectors, selected for high efficiency.
Analog accumulation (AAC) and hybrid photon counting (HPCM) modes.
Excellent for quantitation and photometric analysis. High S/N for imaging of low intensity samples.
Filter Selection Ion deposition filters. 6 filter positions available for each excitation, spectral and emission filter turret. Automated filter selection through software.
Spectral System
Two detection channels, each equipped with an independent diffraction grating and slit for spectral separation and high-speed bandwidth selection.
Selectable wavelength range: 1-100nm per channel
Wavelength resolution: 2nm
Wavelength change speed: 1ms/100nm
Microscopes Inverted: IX81
Upright: BX61, BX61WI
Optical Path Motorized Selection of Optical Path: LSM / Reflected Light Illuminator / SIM Scanner
Anti-vibration Air anti-vibration table
Transmitted Light Detection Built-in transmitted light detector and halogen light source, connected to microscope via fiber cable. Motorized exchange between halogen light illumination and transmitted laser light detection.
External Fluorescence Illumination External fluorescence light source, connected to microscope via fiber cable.
Built-in automated shutter. Motorized exchange between LSM and fluorescence illumination.
PC Control Unit PC-AT compatible
OS: Windows XP Professional
CPU: Pentium 3.6GHz or higher
Memory: 3GB or larger
Hard Disk: 300GB or larger
Special I/F board (built-in PC)
Graphic Board: ATI RADEON X1800
Memory Media: built-in DVD-R/RW
Monitor: two (2) 21-inch monitors standard, single monitor configuration optional
Light Path Options SIM Scanner For photoactivation or photobleaching by second, independent scan unit.
Scanning device: 2 galvanometer mirrors (point scanning), built-in laser shutter.
Standard configuration: single laser port for 405nm Laser Diode or 488nm Argon laser.
Application software and power supply unit required.
4th Channel PMT
Detector Unit
Directly couples an independent PMT detector to basic scan unit. Increases detection capability of basic scan unit to 4 internal detectors for fluorescence + 1 external detector for transmitted light.
Fiber Port Fiber port for fluorescence output to fiber optic.
Equipped with FC connector, compatible fiber core 100-125m.
Principal Software Functions Software Basic acquisition software, application and analysis software.
Image Format Olympus Image format, JPEG, BMP, TIFF and AVI movie format
8-bit and 16-bit gray scale
24-bit, 32-bit and 48-bit full color
Image Acquisition Region designation: point, line, free line, clip, clip zoom
2-dimension: XY, XZ, XT, and *X
3-dimension: XYZ, XYT, XZT, *XY, *XT and *XZ
4-dimension: XYZT, *XZT and *XYT XYTλ
5-dimension: XYZTλ
Real-time image calculation: Kalman filtering, peak detection
Time series controller, protocol processor
*Spectral system only
Image Display Display: Single-channel, side-by-side, merge, live tiling, scan parameters
LUT: Individual color setting, pseudo-color
Overlays: graphic and text input
3D Visualization and Observation 3D animation, cross sectional display, volume rendering
Fluorescence Separation Fluorescence separation through spectroscopy: normal and blind modes
Spectral system only
Image Processing Individual filters: average, low-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement, etc. Calculations: inter-image, mathematical and logical, DIC background leveling, real time ratio/concentration display.
Image Analysis Overview of fluorescence intensity, length, area and perimeter measurement, time-lapse measurement, FRET - Accepter photo bleach and Sensitized FRET analysis
Statistical Processing 2D data histogram display, co-localization
Others (options) Time course software, review station software, XY motorized stage control software
Power Consumption Microscope: 6Amps
Scan Unit: 6.2 Amps
Computer: 4.5 Amps
Multi-line Argon laser: 100Volts, 10Amps
HeNe laser: 0.5 Amps each
Diode Laser: 1.0 Amps each
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