FluoView 1200 Confocal Microscope

Optimized for Imaging Live Cells and Tissues, FV1200 brings sensitivity and power to your research through a range of innovative technology and sensitivity improvements, allowing you to capture the most critical elements of your biological samples with speed, precision and reliability.

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Overview

Imaging of living tissues demands the highest levels of sensitivity which allows for reduced laser power, phototoxicity and photobleaching, and innovative approaches to measurement of fluorescent indicators. Building on Olympus' renowned optical design capability, the FV1200 offers increased laser selection, cooled high sensitivity detector technology, increased scanhead light throughput and new software features designed for high speed functional measurements, and capitalizes on the advantages of the recently introduced IX83® automated inverted microscope platform. Enhancements in detector and coating technology allow for up to 5 simultaneous fluorescent detection channels with 748nm laser excitation for near-IR applications. In addition, the FV1200 offers truly synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1200 an ideal choice for FRAP, FLIP and photoactivation.

THE NEW IX83ZDC MICROSCOPE

  • Real time Z-drift compensation with new ZDC3
  • Greater frame stability and flexibility
  • Touch panel control for easy switching of observation conditions
 

IX83 Zero Drift Control

  • Capable of both continuous and 1 shot autofocus
  • Focus search through cell safe near-IR laser
 

Increased Frame Stability with new IX83

 

HIGH SENSITIVITY GaAsP DETECTORS

  • Hand selected GaAsP with quantum efficiency up to 45%.
  • Combined with Peltier cooling for maximum signal-to-noise detection.
 

Standard PMT

GaAsP
 

Comparison of standard PMT vs GaAsP detector on Olympus FV1200. Signal to noise is improved through a combination of both higher sensitivity and lower dark noise, allowing for greater clarity of images and reduced laser power for live cell imaging. Greatest improvements will be seen with dim samples/regions. Red arrows indicate synapsin signal made clear by GaAsP. Both images were acquired at 0.4% 488nm laser power.

 

High Reflection Silver Coated Scanning Mirrors

  • Anti-oxidization silver coating ensures durability and improves reflection efficiency in both excitation and emission increasing the reflectance in the visible range by 5 - 15%, and IR reflectance up to 22%.
 

HIGH SPEED OPTOGENETICS and UNCAGING: The freedom to choose your own path

 
  • Standard raster scanning modes are not capable of measuring high speed physiology with high signal-to-noise. Olympus’ innovative Multipoint Mapping Advanced Software (MMASW) provides a solution for the most demanding high speed measurements. Using methods similar to Random Access Scanning and Targeted Path Scanning, users have the freedom to choose their own rapid multipoint measurement or stimulation paths.
  • Each scanned position can be expanded to a larger area.
  • Silver galvanometer mirrors drive the scan, ensuring access to full field of view and multicolor scanning capability with excellent light throughput for both visible and IR lasers.
  • Measure cell network fluctuations at up to 101 cycles per second, with up to 50,000 Hz data output per position. High signal-to-noise integration times to capture the data you need.
  • Use the Mapping feature to quickly identify fluorescence fluctuation positions or areas of high signal and automatically assign positions for high speed measurements.
  • Map cellular responses to simultaneous stimulus with Olympus’ unique SIM scanner for optogenetics or uncaging experiments.
  • Synchronize detection with electrophysiology or other external devices.
  • High speed electrophysiology, calcium measurements, optogenetics and FRAP/FLIP experiments. SIM scanner stimulation synchronized with imaging ensures cell responses during or immediately following stimulation are captured as they happen.
  • The stimulation/imaging positions and laser wavelengths can be set separately with two independent beams.
 

High-precision VBF FOR SPECTRAL UNMIXING

High-precision VBF

  • The FV1200 uses a Variable Bandpass Filter (VBF) for maximally utilizing the dispersion of the fluorescence emission to capture freely chosen wavelength ranges. VBF can be opened to 100nm and adjusted in 1nm increments for high sensitivity spectral detection
  • Separation of fluorescent cross-talk is achieved using the lambda scanning and unmixing functions.
  • FV1200 allows combination of spectral detection, filter detection with normal and GaAsP detectors for great flexibility
 

Quantification

  • Laser power monitoring and advanced laser intensity feedback system for stable excitation and stimulation.
  • Diffusion measurement package (DMP) and FRET analysis software allow you to get the most from your imaging. Simultaneous stimulation capabilities for the most demanding dynamic experiments.
  • Re-use acquisition parameters for reproducible experiments. Measurement of fluorescent intensity during observation using live-plot.
 

High Sensitivity

  • Exclusive Analog Accumulation Circuit (AAC) for maximal sensitivity.
  • Uniquely coated filters and dichromatic mirrors enhance sensitivity.
  • Highly sensitive photomultiplier selected specifically for the FV1000.
  • Superior optics for maximal light collection.
 

EXCLUSIVE OLYMPUS OPTICS FOR COLOCALIZATION AND LIVE CELL IMAGING

  • Olympus expands its superior line of live cell imaging optics with 30x, 60x and now 40x silicone oil objectives for optimal refractive index matching and long term time-lapse
  • Super corrected 60x objective for maximal chromatic correction for high resolution colocalization experiments
  • Wide range of UIS2 optics for a broad range of experimental needs
  • Click here for the complete Olympus Objectives collections.
 

Features

Multilaser Combiner

A comprehensive line-up of lasers compatible with a wide variety of fluorescent pigments

Diode Lasers Encompassing Wide Wavelength Range

The Olympus multi-combiner system can be equipped with diode lasers covering 405nm to 635nm wavelength range. This enables efficient observation of the entire palette of fluorescent proteins. (See spectra below.)

The combination of a 473nm and a 559nm laser is optimal for combinations of EGFP and commonly used red fluorophores. (See below for example images.)

Diode lasers have numerous advantages, including improved stability, reduced heat and noise and reduced operating cost due to low power consumption.

Spectra of commonly used fluorophores.
Spectra of commonly used flurophores

Advantage of 473nm and 559nm diode lasers.
Advantage of 473nm and 559nm diode lasers

UPLSAPO series aberration compensation.

 
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SIM Scanner

Learn more about high speed measurements and stimulation with the Multipoint Mapping Advanced Software (MMASW)

The simultaneous (SIM) scanner system allows for simultaneous laser light stimulation and imaging.

Stimulus setting

Stimulus setting

The FV1200 enables selection of the laser wavelength, intensity, scanning mode and other parameters for the stimulation laser, as shown in the dialog box here. The SIM scanner is synchronized with microsecond accuracy.

Assured capture of reactions immediately following laser light stimulation

The compact design incorporates two laser scanners, one for confocal imaging and the other for simultaneous stimulation. They can be illuminated separately and independently, making it possible to stimulate the specimen during observation. As a result, the rapid cell reactions that occur right after laser stimulation can be accurately and reliably captured, making the FV1200 ideal for such applications as FRAP, FLIP, photoactivation and photoconversion. (See Applications tab for details on each method and how the FV1200 improves results.)

Stimulation area can be changed during imaging

Two laser beams, one for imaging and the other for laser light stimulation, can be independently controlled. The stimulation area can be moved to a different position on the field of view during imaging, allowing multiple stimulation events.

Wide choice of different bleaching modes

Various scan modes can be used for both the observation area and stimulation area. This enables free-form bleaching of designated points, lines, free-lines, rectangles and circles.

Multi-combiner enables laser output to both scanners

Light from multiple lasers is precisely combined and transmitted by a branched fiberoptic so that all wavelengths can be used on both scanners for imaging and stimulation.

Tornado Scanning

Tornado scanning for highly efficient bleaching Photostimulation, Photoactivation

Conventional raster scanning cannot always complete photobleaching quickly. Beam blanking during mirror turnaround reduces efficiency in raster scanning. Tornado scanning makes the procedure much more efficient by providing continuous laser illumination and significantly reducing unnecessary scanning.

Imaging the immediate effects of light stimulation

Laser light stimulation can change the intensity or emission wavelength in certain fluorescent proteins and newly developed fluorophores. Such optical highlighting allows pools of labeled molecules to be monitored in live cells or organisms. The FV1200's SIM system can provide stimulation scanning separately from imaging scanning, for simultaneous stimulation and imaging the response at different locations in the cell or organism.

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Spectral and Filter Scan Units

Filter Scanhead

Spectral Scanhead

The spectral and filter scan units provide three types of high performance image capture.

VARIABLE BANDPASS FILTER (VBF)

With the spectral detection system, once a fluorochrome combination is selected within the software, the ideal range of emission wavelengths is automatically selected. Manual adjustment of the wavelength range is also possible for two detectors according to the fluorescence emission spectrum. The variable barrier filters can be used to optimize image acquisition since the sensitivity of each channel can be adjusted independently.

High-performance filters

High sensitivity gallium arsenide phosphide (GaAsP) detector unit

High-performance anti-reflective coatings are used on all emission filters and dichromatic mirror units, achieving sharper transitions which were not possible with the conventional vacuum deposition method. (See graph.) In particular, these mirrors cover the full fluorescence emission spectrum for fluorescent proteins, enabling clear observation even with weak fluorescence emission while minimizing damage to live cells.

High performance filters

High-sensitivity detection system

High-sensitivity, high signal:noise ratio optical performance is achieved through integration of a pupil projection lens within the optics and employment of a high-sensitivity photo multiplier and analog processing circuit with minimal noise. Even greater sensitivity is provided with GaAsP detector unit.

 Standard Quantum Efficiencies of Detector Technologies Comparison of galvano mirror Silver vs Aluminum
 

Photon counting detection mode

The Olympus original Hybrid Photon Counting Mode (HPCM) can successfully capture images even when fluorescence emission is weak. This mode optimizes photo multiplier control conditions to acquire images with minimum noise and high quantitative performance.

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Software

The FV1200 ASW software provides many key features to enable your research.



Suitable for multiple users

For the convenience of multiple researchers, each user may create an independent log which contains individual settings such as the display window and toolbar. To change researchers, the new user simply logs in at software start-up.

Wide choice of scanning modes

Several scanning modes are available, including ROI, point and high-speed bi-directional scanning. These can be used together freely, in such combinations as XYZ, XYT, XYZT, XYλ, XYλT, etc.

Easy designation of scan area

The observation field of view and scanning area are displayed graphically, and settings can be altered while adjusting magnification with the scrollbar. The operator can move the image scan area at will using the Pan button. Rotation scanning of the image field is also possible.

Frame and Line Sequential Excitation

Eliminate cross talk by using both frame and line sequential modes. The order of image acquisition, or image combinations, can be freely changed.

Free emission wavelength selection

When selecting fluorochromes from within the software, the fluorescence spectra are displayed and the ideal detection wavelengths are automatically selected. Manual adjustment of the wavelength emission range, in as little as 1nm step increments, is also possible in order to optimize acquisition of fluorescence emission.

LARGE FILE ACQUISITION CAPABILITIES

Direct disk access allows for acquisition and opening of single Z stacks over 150GB, and multi-area acquisitions 100s of GB in size.

Microscope Controller
Microscope Controller

On-line help

The comprehensive on-line help explains each command's function and usage, as well as the overall flow of operations.

One-touch change between confocal and fluorescence observation

Since the FV1200 is fully automated, one-touch changes observation from confocal laser scanning to ocular viewing of widefield fluorescence. In addition, microscope settings can be easily changed through the Microscope Controller.

Easy image acquisition for 3D, 4D and 5D series

Multi-dimensional image acquisition, such as λ series, Z series and time-lapse, is easily performed.

Automatic contrast

Automatic contrast selects optimal photomultiplier voltage settings for each detection channel, simplifying image acquisition.

Real-time emission intensity graph display

For image acquisition in real time, a live plot of emission intensity is displayed in a graph. Since the images are captured using the full 12-bit capacity, this is also convenient for setting sensitivity levels.

2D View Analysis Tool

background correctionBackground Correction:
Subtracts background.

Region MeasurementRegion Measurement:
Measures size or intensity of a designated region of interest (ROI).

Intensity ProfileIntensity Profile:
Displays intensity profile of region designated with ROI or Line.

HistogramHistogram:
Displays histogram of intensity values of a designated region of interest (ROI).

Diffusion Measurement PackageDiffusion Measurement Package:
Measure molecular concentrations using Raster Integrated Correlation Spectroscopy (RICS), ccRICS, point FCS and other methods.

Series AnalysisSeries Analysis:
Analysis of variation in intensity along z-axis/time-axis in a designated region of interest (ROI).

Line Series AnalysisLine Series Analysis:
Analysis of variation in intensity along z-axis/time-axis on a designated line.

ColocalizationColocalization:
Analysis of degree of overlap between two fluorescent labels. Pearson's co-efficient and Manders' Overlap are calculated using thresholds.

RatioRatio:
Create an image using the intensity ratio between two channels.

Multipoint Mapping Advanced SoftwareMultipoint Mapping Advanced Software:
High speed fluorescent measurements at 101Hz with 50,000 Hz output per position. Map cell responses to light or electrical stimulation.

 

Easy image search

Explorer software provides simultaneous thumbnail image display, making it easy to search for previously stored data.

Data manager

The data manager provides thumbnail displays and different types of file information.

2D image display

The 2D control panel enables free manipulation of the image display. Tiling display or multi-dimensional image display can also be freely selected.

Re-use function

Previously-set scanning conditions can be recalled and applied to new or subsequent experiments.

File Input/Output

OIB (Olympus Image Binary format) is employed to acquire both scanning and microscope setting conditions together with image acquisition data. This software also handles all widely used image formats (TIF, BMP, JPEG, AVI, MOV etc) with high interchangeability.

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