MetaMorph For Olympus Packages
The MetaMorph for Olympus is available for both acquisition and offline (analysis only) needs.
METAMORPH FOR OLYMPUS ACQUISITION FEATURES
- Drivers for many popular CCD cameras, Olympus IX2® and BX3® motorized microscopes plus shutters, filterwheels, and XY stages from various manufacturers
- Multi-dimensional acquisition interface
METAMORPH FOR OLYMPUS ACQUISITION OPTIONS
- Simultaneous and split camera acquisition
- Automated slide scanning and stitching
- Multiwell plate interface
- Control of various devices for FRAP, uncaging, photobleaching and photoablation devices
METAMORPH FOR OLYMPUS ACQUISITION AND OFFLINE FEATURES
- Multi-dimensional data display interface
- Montage display
- Image processing features such as shading correction and kernel filters
- Integrated Morphometry Analysis for detection and analysis of objects
- Colocalization and FRET tools
METAMORPH FOR OLYMPUS ACQUISITION AND OFFLINE OPTIONS
- 4D viewer
- Motion tracking
- Multiwell data viewing
- Application Modules for specialized application analysis needs
Additionally, we offer MetaFluor for Olympus for ratio imaging of intracellular ion concentrations.
For product and sales information, please contact: metasales@olympus.com
MetaMorph and MetaFluor® are trademarks of Molecular Devices, Inc.
Application Modules
MetaMorph for Olympus offers eleven user-friendly Application Modules for
biology-specific analysis. Each Application Module features a dedicated dialog box
with intuitive setting selections, improved segmentation through adaptation to local
content, and both field and cell-by-cell data logging. After analysis is complete,
users can interactively view tabular and image results side by side. Application Modules
may be incorporated into macros for increased customization and automation of analysis.
All Application Modules are validated in-house and with third-party collaborators.
Available Application Modules are:
A number of different Application Modules can be used to analyze data from common experiments such as:
- Endothelial tube formation (Angiogenesis)
- Promotion or inhibition of blood vessel formation (Angiogenesis)
- Cell viability (Live/Dead, Cell Cycle, Cell Health, Multi Wavelength Cell Scoring)
- Cell cycle (Mitotic Index, Cell Cycle)
- Membrane potential (Cell Health)
- Cytotoxicity and apoptosis (Live/Dead, Cell Health)
- Cell proliferation (Count Nuclei)
- Cell migration (Count Nuclei)
- Cell counting (Count Nuclei)
- Kinase activation (Cell Scoring)
- Fatty acid uptake (Cell Scoring, Multi Wavelength Cell Scoring)
- Mitosis (Mitotic Index, Cell Cycle)
- Adipogenesis (Cell Scoring, Multi Wavelength Cell Scoring)
- Examining transfection efficiencies (Cell Scoring, Multi Wavelength Cell Scoring)
- Studying intracellular structures (Granularity)
- Receptor internalization (Granularity)
- Clustering target molecules(Granularity)
- Process extension (Neurite Outgrowth)
- Neurodegenerative or neuroregenerative diseases (Neurite Outgrowth)
- Cell differentiation (Stem cell research) (Neurite Outgrowth)
- Protein expression & modification (Cell Scoring, Multi Wavelength Cell Scoring)
- Transient transfection (Cell Scoring, Multi Wavelength Cell Scoring)
- Detect monopolar spindles (Monopole Detection)
- Cell signaling (Cell Health, Cell Cycle)
Angiogenesis Tube Formation Application Module
- Better quantitation by creating a single in-focus composite image from multiple Z-series images
- Multi-parameter analysis measurements include tube length, number of branch points, number of nodes and more
Figure 1. Left: a best focus image is obtained from a Z series, right: The application module identifies tubes (white) and nodes (green).
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Cell Cycle Application Module
- Classification and quantification of cells in different stages of the cell cycle
- Option to use specific apoptosis and mitotics stains, for increased classification accuracy
Figure 2. Cell cycle classification can be performed with a single or multi wavelength assay. Left: CHO-K1 cells stained with Hoechst 33342. Right: The Cell Cycle module identifies cell cycle phases: G0/G1 (dark blue), S (light blue), G2 (green), Early M (orange) and Late M (red).
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Cell Health Application Module
- Analysis of up to three fluorescent probes for cell-based apoptosis and necrosis assays
- Classification and quantification of viable, early and late apoptotic cells, necrotic cells
Figure 3. Adherent Chinese Hamster Ovary (CHO-K1) cells were incubated with various concentrations of staurosporine for 6-12 hours. Top left: control, top right: 0.1 µM staurosporine, bottom left: 3 µM staurosporine, bottom right: measured (Green: viable, blue: early apoptotic, purple: late apoptotic, red: necrotic).
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Cell Scoring Application Module
- Identification of two sub-population of cells
- Ideal for counting and logging measurements of cells in two-wavelength experiments
Figure 4. The module can be used as a segmentation tool to identify two different stains or to score cells.
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Count Nuclei Application Module
- Automatically counts nuclei and captures intensity measurements
- Accurate segmentation of touching cells
Figure 5. The Count Nuclei module identifies cells as unique objects.
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Granularity Application Module
- Count punctuate objects
- Designed for receptor internalization or clustering target molecules
- Choice of six granularity indices
- Measurements include count, total and mean area, and intensities of granules and nuclei
Figure 6. U2OS cells, top: overlay, bottom: the module identifies granules and nuclei, even in cells with high background (red arrow).
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Live/Dead Application Module
- Classification and quantification of live and dead cells
- Measurements include counts and percentages of live and dead cells, per-wavelength measurements and more
Figure 7. CHO-K1 cells incubated with DMSO for 6-12 hours prior to staining. Cells were labeled with H33342 and PI diluted in PBS for 30-60 minutes before image acquisition. Top left: control, top right: 20% DMSO, bottom: measured (red: dead, green: live).
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Mitotic Index Application Module
- Classification and quantification of mitotic and interphase cells
- Measurements include count, percentage and wavelength-specific intensities of mitotic and non-mitotic nuclei, and more
Figure 8. Left: CHO-K1 cells treated with Nocadazole for 18 hours before staining with anti-phospho-Histone H3 (Ser28). Right: mitotic (green) and interphase (red) cells.
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Monopole Detection Application Module
- Classification and quantification of mitotic cells with monopolar or bipolar spindles
- Measurements include count and percentage of monopoles, bipoles and interphase cells, DNA structures area, cell classification and more
Figure 9. 3T3-L1 mouse fibroblast cells treated with monastrol and stained with mouse anti-beta tubulin primary antibody detected with a FITC conjugated goat anti-mouse secondary antibody. Nuclei are stained with Hoeschst 33342. Left: monastrol, right: segmented image shows interphase cells (red), bipolar spindles (blue) and monopole (green).
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Multi Wavelength Cell Scoring Application Module
- Multi-parametric analysis of up to seven wavelengths
- Identification of sub-populations of cells
- Measurements include scoring profiles, wavelength-specific count and percentage of negative and positive cells, cell-by-cell wavelength-specific stained area, integrated and average intensities.
Figure 10. Top: two wavelength images, bottom left: overlay, bottom right: segmentation.
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Neurite Outgrowth Application Module
- Designed for the measurement and analysis of neurite outgrowths
- Works with or without nuclear stain
- Measurements include total neurite outgrowth, total branches and cell bodies, straightness and more
Figure 11. Left: PC-12 cells, middle: the module identifies cell bodies and outgrowths, right: segmentation image.
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- Kinase activation (Cell Scoring)
- Fatty acid uptake (Cell Scoring, Multi Wavelength Cell Scoring)
- Mitosis (Mitotic Index, Cell Cycle)
- Adipogenesis (Cell Scoring, Multi Wavelength Cell Scoring)
- Examining transfection efficiencies (Cell Scoring, Multi Wavelength Cell Scoring)
- Studying intracellular structures (Granularity)
- Receptor internalization (Granularity)
- Clustering target molecules(Granularity)
- Process extension (Neurite Outgrowth)
- Neurodegenerative or neuroregenerative diseases (Neurite Outgrowth)
- Cell differentiation (Stem cell research) (Neurite Outgrowth)
- Protein expression & modification (Cell Scoring, Multi Wavelength Cell Scoring)
- Transient transfection (Cell Scoring, Multi Wavelength Cell Scoring)
- Detect monopolar spindles (Monopole Detection)
- Cell signaling (Cell Health, Cell Cycle)
MetaMorph and MetaFluor® are trademarks of Molecular Devices, Inc.
MetaFluor® for Olympus
Fluorescence ratio imaging is the monitoring of live cells in which a fluorescent indicator
of intracellular ions is introduced. Indicator dyes have been designed to shift their fluorescence
excitation or emission spectrum when binding with specific ions. Images are obtained at two
different wavelengths, typically matching the absorption bands at the high and low binding conditions.
By rationing the intensities in the images, it is possible to construct a map showing the
local ion concentrations throughout the field of view. Since the monitoring process is nondestructive,
image acquisition can be repeated frequently to trace and monitor the time course of cellular responses.
MetaFluor® for Olympus is designed for dual-wavelength intracellular ion measurements. The system
provides simultaneous display of the raw data, ratio image, graphs of intensities, ratios and ion
concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different
ratiometric indicators can be imaged and measured simultaneously.
Custom Configuration
Toolbars, menus, wizards and dialog boxes help move you through the image
processing steps quickly. Features such as multiple image windows, flexible
device control, synchronization and timing, and journals allow for automated
image acquisition and analysis unlike any other system.
With MetaFluor for Olympus, you customize the set-up once, then let the experiment run
by itself. You are able to collect a large amount of data online and process
it with either MetaFluor® for Olympus or an analysis-only copy of the software.
Monitoring of Live Cells
Regions of interest can be generated automatically or manually placed on
your image to monitor intensity, ratio value or ion concentration.
Measurements are then made simultaneously on all the regions of interest
and update continuously on a scrolling graph, allowing you to follow
dynamic changes as they occur in your living samples.
A display of multiple graphs gives flexibility in the presentation of your
experiment''''s data. MetaFluor enables you to click on graph traces to
display a readout of the time and data value for the region nearest to
the click.
The Event Mark function is useful to record when drugs or solutions were
added, experimental conditions changed, triggers were received or sent or
other events occurred. You have the option to associate a timer and an
alarm bell to each event. Additionally, for perfused samples, ambient
conditions can be logged and tracked. Each image has an annotation that
is saved within the TIFF file format. The annotation will record
wavelength-dependent settings. Additional information can be stored
in a protocol file.
Export Data for Analysis
If needed, MetaFluor for Olympus can log and export all measurements to either a text
file or to a spreadsheet program such as Microsoft® Excel.
Compatible with MetaMorph for Olympus
Because MetaFluor for Olympus saves images in TIFF file format, you can import them into
MetaMorph for Olympus for further processing and analysis.
Presentation and Publication
Images in MetaFluor for Olympus can be displayed in monochrome, pseudocolor, or using a
variety of user-defined set of values. Ratio images can also be displayed
using a special display mode called Intensity Modulated Display, or IMD.
With the IMD mode, color is used to represent the relative ratio value,
while the intensity or brightness of the color is used to represent whether
the brightness of the source images. This technique helps automate the
process of extracting spatial information from the background, by automatically
eliminating background fluorescence from the scene.
MetaMorph and MetaFluor are trademarks of Molecular Devices, Inc.
