Applications - VivaView® FL Incubator Fluorescence Microscope

The VivaView FL provides a fully integrated and motorized inverted microscope to allow high quality, long-term time-lapse imaging in a constant and optimized environment. Multiple locations in up to 8 samples can be imaged simultaneously with fluorescence or Differential Interference Contrast. Simple, intuitive computer operation removes all the difficulty previously associated with configuring a live cell imaging system. The VivaView FL is the next generation in live-cell imaging providing researchers the ability to image cells for significant longer than has previously been possible with absolute control of the surrounding environment.

VIVAVIEW FL INCUBATOR FLUORESCENCE MICROSCOPE APPLICATIONS

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PUBLICATIONS

Meeting the Challenges of Long-term Time-lapse Imaging - Bioscience Technology Sept 2009 (PDF) Read More >
Takahiro Seki, Takayuki Shimahara, Kazuhiro Yamamoto, Nana Abe, Taku Amano, Naoko Adachi, Hideyuki Takahashi, Kaori Kashiwagi, Naoaki Saito, Norio Sakai. (2009). Mutant ?PKC found in spinocerebellar ataxia type 14 induces aggregate-independent maldevelopment of dendrites in primary cultured Purkinje cells. Neurobiology of Disease. Vol. 33, 260-273. Read More>
Asako Sakaue-Sawano, Hiroshi Kurokawa, Toshifumi Morimura, Aki Hanyu, Hiroshi Hama, Hatsuki Osawa, Saori Kashiwagi, Kiyoko Fukami, Takaki Miyata, Hiroyuki Miyoshi, Takeshi Imamura, Masaharu Ogawa, Hisao Masai, and Atsushi Miyawaki. (2008). Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression. Cell. Vol. 132, 487-498. Read More>
Asako Sakaue-Sawano, Kenji Ohtawa, Hiroshi Hama, Masako Kawano, Masaharu Ogawa, and Atsushi Miyawaki. (2008). Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology. Vol. 15, 1243-1248. Read More>
Hiroki Umeshima, Tomoo Hirano, and Mineko Kengaku (2007). Microtubule-based nuclear movement occurs independently of centrosome positioning in migrating neurons.
PNAS. Vol.104, No. 41, 16182-16187. Read More>
Takahiro Hatanaka, Yasue Hatanaka, Jun-ichi Tsuchida, Vadivel Ganapathy, and Mitsutoshi Setou. (2006) Amino Acid Transporter ATA2 Is Stored at the trans-Golgi Network and Released by Insulin Stimulus in Adipocytes. Journal of Biological Chemistry. Vol. 281, No. 51, 39273-39284. Read More>

VIDEOS

Screening drugs to block aggregation of Huntingtin protein

Apoptosis: An award-winning video

Instrument: VivaView FL (LCV-110)

Subject: This video is a series of live, time-lapse images of human mesenchymal stem cells grown on a collagen-I coated dish. The cells are treated with a high level of a PI3-Kinase inhibitor which induces apoptosis (or programmed cell death). This video won an award at the 2010 American Society for Cell Biology Cell Dance competition.

Reference: Video courtesy of Ms. Kira Henderson, PhD student, Department of Biology, Rennselaer Polytechnic Institute, Troy, NY.

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Screening drugs to block aggregation of Huntingtin protein

Instrument: VivaView FL (LCV-110)

Subject: Montage displaying aggregate accumulation of GFP-tagged truncated Huntingtin in PC12 cells under 3 different concentrations of an aggregation inhibitor drug vs. control media.

Reference: Emily Mitchell, Leslie Thompson, Ph.D., University of California, Irvine

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Human neural stem cells are healthy during two weeks of imaging

Instrument: VivaView FL (LCV-110)

Subject: 2 week time lapse of Human Neural Stem cells transfected with GFP CAG. Development is followed after the addition of differentiation media at T = 24 hours.

Reference: Chris Sontag, Brian J. Cummings, Ph.D., University of California, Irvine

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Comparing effects of immunosuppressant drugs on human neural stem cell fate

Instrument: VivaView FL (LCV-110)

Subject: Immunosuppressant drug study of Human Neural Stem cells to analyze proliferation and migration. Movie shows a 2 x 2 montage from a single specimen dish. Focus is maintained throughout as new drug and media is added every 3 days.

Reference: Chris Sontag, Brian J. Cummings, Ph.D., University of California, Irvine

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Migration tracking of human neural stem cells

Instrument: VivaView FL (LCV-110)

Subject: Tracking data of Human Neural Stem cells captured at 15 frames per second.

Reference: Chris Sontag, Brian J. Cummings, Ph.D., University of California, Irvine

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Biocompare Video for VivaView FL Incubator Fluorescence Microscope

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Hela Cells constantly-expressing Fucci

Instrument: VivaView FL (LCV-110)

Subject: VivaView FL makes a contribution as the microscope to provide a stable environment in culture for the long term. 64 hours 13.5 minutes.

Reference: Sakaue-Sawano A, Atsushi Miyawaki M.D., Ph.D. RIKEN Brain Science Institute, Laboratory for Cell Function Dynamics

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HeLa Cells expressing Histone H2B-GFP Cells divide 3 times during 60 hours of fluorescence and DIC observation

Instrument: VivaView FL (LCV-110)

Subject: Successful observation of 3 divisions in cells expressing GFP localized to the nucleus is challenging for fluorescence microscopy. The VivaView FL allows long-term imaging by minimizing damage to the cells while maintaining image qality.

Reference: Atsushi Miyawaki M.D., Ph.D. RIKEN Brain Science Institute, Laboratory for Cell Function Dynamics

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Purkinje neuron

Instrument: VivaView FL (LCV-110)

Subject: Fluorescence time lapse observation of primary cell cultures is extremely difficult. Using the VivaView FL, Dr. Kengaku successfully captures a 4 days time-lapse data series of a neuron.

Reference: Mineko Kengaku Ph.D., RIKEN Brain Science Institute, Laboratory for Neural Cell Polarity

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