FV1200MPE TWIN

The FV1200MPE TWIN is the ultimate confocal and multiphoton system for optogenetics, electrophysiology and high speed measurements in live tissues. With dual IR laser inputs, an increase of 50% in multiphoton excitation efficiency and superb MPE dedicated objectives, the FV1200MPE TWIN delivers high sensitivity and deep imaging with the ability to simultaneously image and stimulate with both pulsed IR and visible lasers. Configured with standard visible confocal capability and a range of descanned and non-descanned detector options, the FV1200MPE twin pushes the envelope for interactive visible and multiphoton imaging.

Product Image

SPECIFICATIONS

B System S System T System M System
Laser unit

IR pulsed laser with negative chirp for multiphoton excitation

•Mode-locked Ti : sapphire laser [femtosecond laser (equipped with a group velocity compensation)], laser power unit, water-cooled circulating chiller
IR pulsed laser can be controlled with the FV10-ASW (ver.2.1 or later) software
•MaiTai BB DeepSee-OL, MaiTai HP DeepSee-OL or MaiTai eHP DeepSee-OL (Spectra-Physics products)
MaiTai BB DeepSee-OL : 710nm — 990nm
MaiTai HP DeepSee-OL : 690nm — 1040nm
MaiTai eHP DeepSee-OL : 690nm — 1040nm (70 femtoseconds at a specimen plane)

Visible light laser AOTF laser combiner

LD laser: 405nm: 50mW, 440nm: 25mW, 473nm 15mW, 559nm 15mW, 635nm 20mW
AOTF laser combiner Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe (G) laser (543nm, 1mW)
Modulation: Continuously adjustable via an AOTF (0.1 — 100% in 0.1% increments)
  • Operating mode: Allows laser turn-off during the retrace period
    REX: adjustment of laser power for each region, and selection of the laser and selection of the laser
    wavelength
  • Visible light laser platform with implemented AOTF system, ultra-fast intensity with individual laser
    lines, additional shutter control, Connected to scanner via single-mode fiber
  • Equipped with laser feedback mechanism to limit changes in laser light intensity over time
N/A
Single laser for visible light LD473 laser (15mW) Depending on the type of modulation: light intensity modulation, shutter control, connected to the scanner via single-mode optical fiber. N/A
Scanning Unit Scanning method Light deflection via 2 falvanometer scanning mirrors
Scanning modes

•Pixel size: 64 x 64 — 4096 x 4096 pixels Scanning speed: (pixel time): 2μs — 200μs High-speed scanning mode: 16 frames/s (256 x 256) •Dimensions: Time, Z, (wavelength) (or any combination thereof)
•Line scan: straight line (includes rotation), free line, point XY scan

Zoom size Observation position zoom with inclination width modification of galvanometer mirror: 1-50x (adjustable in 0.1x increments.
Confocal detector (The M scanner does not have a confocal detector)
  • Detector: 3 channels for fluorescence detection (photomultipliers), (The M scanner does not optional 1CH detector for expansion have a confocal detector)
  • Dichromatic mirrors for excitation, dichromatic mirrors for multiphoton excitation, dichromatic mirrors for fluorescence, emission filter
  • Infrared cut filter: using a high-performance filter
  • A filter or spectral type of fluorescence detector can be selected
  • Spectral type: Channels 1 and 2 provided with independent grating and slit
  • Selectable wavelength range: 1 – 100 nm, wavelength resolution: 2 nm, wavelength switching speed: 100 nm/ms
  • Pinhole: Single motorized pinhole, pinhole diameter: ø50 – 800μm (spectral type ø50 – 300μm), adjustable in 1 μm increments
  • Field Number: 18
N/A
Optics with infrared laser for multiphoton imaging
  • Integrates a multiphoton near-infrared pulsed laser in the scanning unit (Laser safety measures implemented)
  • Continuously variable output using AOM (1 – 100%, 0.1% increments)
Component incorporating the laser for multiphoton imaging
Main scanner for observation
ASU scanner for laser light stimulation, Main Scanner for observation: VIS laser
Incorporating 2 independent lasers for laser light stimulation/observation
M scanner for observation
Detector for multiphoton imaging Reflected light fluorescent detector Photomultiplier (2 or 4 channels), Fluorescence wavelength can be selected with teh dedicated filter cube (replaceable)
Transmitted light fluorescent detector Photomultiplier (2 channels), fluorescent detector Fluorescence wavelength can be selected with the dedicated filter cube (replaceable)
Exclusive equipment for the BX61WI upright microscope
Transmitted DIC unit
  • Integrated transmitted light detector and transmitted illuminator, Motorized switching
    Connected to microscope via fiber cable (IR-DIC observation using an infrared laser is not possible)
Z-drive
  • A motorized focus module inside the microscope is used
  • Minimum increment: 0.01μm
Microscope Upright microscopes: BX61WI, BX61 Inverted microscope: IX83
System control
  • OS: WindowsXP Professional (English version), WindowsVista (English version)
  • CPU: Core2Duo 2.33GHz •Memory: 2.0GB or larger
  • Hard disk: 320GB or larger •Dedicated I/F board: built-in PC •Graphics board: Open GL-compliant
  • Recording media: Equipped with DVD dual drive
Required installation environment Room temperature: 20 – 25°C, humidity: 75% or less@25°C, requires continuous (24-hour) power supply
Vibration isolation table for microscope and laser installation, size
1500mm x 1250mm
1500 mm x1500 mm
1700 mm x1700 mm
1500mm x 1250mm

* All other third party marks are property of their respective owners.


OVERVIEW

  • New anti-corrosive silver galvos increase multiphoton excitation efficiency by 50%.
  • Supporting new Spectra Physics® Insight® laser for transmission out to 1300nm*.
  • Dual simultaneous multiphoton wavelength excitation imaging available.
  • High sensitivity hand-selected GaAsP detect or option with quantum efficiency up to 45%.
  • Award winning 4mm and 8mm SCALEVIEW 25x MPE dedicated objectives for the deepest imaging of fixed samples.
  • High speed measurements up to 101 cycles per second and 50,000 Hz readout with MMASW software.
  • A less complex optical path with only two alignment points.
  • Newly designed delivery system for optimal performance with use of the Spectra Physics® MaiTai® DeepSee® Laser, specifically tuned to match Olympus’ optical requirements.
  • Olympus exclusive FastScan high speed IR laser tuning with Olympus version of MaiTai® lasers.
  • Two or four Channel Non-descan Detectors.
  • BX2 channel transmitted detector designed for high sensitivity detection for both transmitted fluorescence and SHG (on the upright platform only).
  • Compact Design – one of the smallest footprints for MPE, 1500mm X 1500mm (60in X 60in).
  • Visible imaging available – 405nm, 440nm, 473nm, 458nm, 488nm, 515nm, 559nm, and 635nm laser lines.
  • Upright or inverted platforms.
  • AOM standard.
  • Dispersion Compensation standard.

Spectra Physics, Mai Tai, and Deep See are registered trademarks of Newport Corporation.
*Insight laser and routing optics are special order and only available in the United States and Canada.

laser safety

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