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A low-cost high-performance accessory for multimodal in vivo imaging without the need for staining protocols or fluorescent protein expression.
The femtoCARS equipped FV1000MPE provides a label-free lipid imaging capability based on molecular vibrations using the Coherent Anti-Stokes Raman Scattering (CARS) microscopy method. CARS is a non-linear optical method with high chemical selectivity and sensitivity that is improved orders of magnitude relative to conventional Raman detection. In Olympus's design, the CARS imaging mode can be combined with two photon excitation fluorescence (TPEF) and second harmonic generation (SHG) imaging modes as a multimodal femtosecond laser imaging station. The femtoCARS add-on provides an entirely new commercial solution for investigating structures such as the myelin sheath, intracelluar lipid bodies, mechanisms underlying fat storage and membrane organization, as well as tissue and skin reorganization in vivo.
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