FluoView FV1200MPE femtoCARS Add-on

The femtoCARS equipped FV1200MPE provides a label-free lipid imaging capability based on molecular vibrations using the Coherent Anti-Stokes Raman Scattering (CARS) microscopy method. In the Olympus design, the CARS imaging mode can be combined with two photon excitation fluorescence (TPEF) and second harmonic generation (SHG) imaging modes as a multimodal femtosecond laser imaging station. CARS is one of a number of intrinsic imaging modes requiring no extrinsic label. Because it relies on molecular vibrations rather than traditional fluorescence, there is no photobleaching.
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  • Simultaneous Lipid (CH2), TPEF, and SHG imaging. The lipid filter set is included and TPEF and SHG filter sets are optional.
  • Uses the standard Olympus DeepSee® lasers which are specifically tuned to match Olympus optical requirements. No additional laser is required.
  • The output of the femtoCARS is pre-set to lipid detection.
  • Standard MPE detection system, up to two forward (transmitted) and four backward (epi) Non-Descanned Detection channels.
  • Standard MPE imaging (by-pass CARS add-on) and single photon imaging are available.
  • The optical delivery system including a laser-safe enclosure is provided and serviced by Olympus America Inc.
  • The overall footprints for MPE with femtoCARS remain small, but different from regular MPE systems.
  • Upright platforms are standard. For inverted platform, please contact Olympus America Inc.
  • Acousto-optical modulation (AOM) standard for MPE and manual power adjustment operation for the Stokes’ beam.
  • Dispersion compensation standard for MPE and for efficient Stokes’ beam generation.

The FV1200MPE femtoCARS is a special order product and only available in US and Canada currently.
Spectra Physics, Mai Tai, and Deep See are registered trademarks of Newport Corporation.

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