Accessories - Andor Revolution XD Spinning Disk Confocal

When it comes to live cell imaging, you need the best optics and a system with tight opto-electronic integration to capture those dynamic events. Olympus and Andor have built reputations for optical excellence and flexible system designs to meet the most challenging research needs. Collaboration between Olympus and Andor means outstanding support and true partnership for your research.

ANDOR REVOLUTION XD SPINNING DISK CONFOCAL ACCESSORIES

MICROSCOPY TECHNIQUE - CONFOCAL DUAL SPINNING DISK

Microscopy technique

Revolution XD: the superior choice for live cell imaging

Spinning disk confocal is ideal when laser scanning confocal speeds are insufficient.
Highly flexible laser combiner allows for the use of up to 6 lasers. Lasers may be added or exchanged to accommodate user needs.
Optical train customized to eliminate internal reflections, hence minimizing background signal.
Precision mounting system for optical train assures optimum alignment and flatness of field for inverted and upright microscopes.
Tight synchronization of Andor laser combiner with Andor time-course studies.
Highly configurable acquisition through MetaMorph^® software.
High frame rates plus piezo Z capability make the Revolution XD a great source for acquiring live multi-color 3D volumes over time.

MICROSCOPY APPLICATIONS

Revolution components and systems benefit from common features across applications. State of the art performance, flexible component-oriented supply and an open-minded approach to systems and technically demanding solutions. Microscopy application

Key Applications

Cell Motility
Live Cell Confocal
FRET Imaging
Ion Signalling
Fluorescent Proteins
Single Molecule
Comet Assay
FISH
Immunofluorescence
Bio/Chemiluminescence

DIVERSE COMPATIBILITY WITH OLYMPUS MICROSCOPES

There are a diverse group of microscopes that are compatible with the Andor system. These microscopes feature IX81^® ZD2, IX71, BX61^®, BX61WI and BX63.

Features

Active high speed focus compensation support
Control microscope motorization
Vibration isolation tables
Stage top and Cage incubators
Options for temperature, humidity and CO2 regulation
Compatible with manual and fully motorized stands even the Olympus Up/Down Microscope

Camera Options

iXon3	Clara	Neo
Single Photon Sensitivity	Resolution & Sensitivity	Drive Optimal Performance

Features Single photon sensitivity and back-illuminated sensors UltraVacTM cooling to -100°C Unique ultrafast Crop Mode OptAcquire one-click optimization Count Convert calibrates in electrons or photons EMCALTM self-calibration of EM Gain DU-897 - The ultimate combination of sensitivity and frame rate DU-888 - 1MP resolution and exceptional sensitivity combined DU-860 - For high speed applications e.g Ca2+ flux	Features Ideal for high-resolution cell microscopy 1.3 megapixel interline CCD; 6.45 μm pixels Very low readout noise (< 3e-) Deep thermoelectric vacuum cooling down to -45°C Broad Vis-NIR wavelength coverage (with enhanced NIR version available) Up to 20 MHz readout speed USB 2.0 connectivity	Features Lower detection limit than any CCD camera 5.5 megapixel sensor format and 6.5 um pixels Minimization of dark current and pixel blemishes Rapid frame time rates

CSU-X1

Confocal Spinning Disk Unit

Features:

1500 - 10,000 rpm disk, producing between 300 to >2000 frames per second
Easy synchronization of exposure to disk speed
Manual and motorized filter changer models available
Optional bright field bypass module
2x excitation throughput improvement compared to CSU10 or CSU22
FWHM Z section < =1.2 μm
Semrock dichroic and emission filter pairing provides optimum Signal to Noise Ratio (SNR)
Filter wheel mounting for fluorescence and DIC analyzer
Andor 10 position filter wheel available
Yokogawa 6 and 12 positions filter wheels

Laser Combiner

Compact, flexible, robust
Opto-electronic control

Features:

Integral AOTF for microsecond laser selection and modulation
Hardware blanking minimizes specimen exposure
Long life solid state lasers with excellent stability (typical ± 0.5% peak to peak)
Up to 6 solid state lasers (from 405, 445, 488, 491, 515,
532, 561, 594, 640 nm)
Powers from 25 - 250 mW depending on wavelength
Compact 4U 19” rack mount enclosure
Quiet, cool with low power consumption

Precision Controller Unit

Flexible Expansion

Precision Controller

Features:

Provides system synchronization
PCUB - Houses AOTF driver for ALC coupling
Optional 16 bit Analog output for direct piezo Z control
8 digital inputs and 8 digital outputs for sensing and triggering external events
Enables Revolution™ to slave or master for complex triggering synchronization
Metamorph^® control

Imaging Workstation

Optimized PC workstation for live cell work
Image Workstation

Handling, processing and visualizing multidimensional images is very computer intensive. The Andor workstation is a high end Desktop PC optimized for use with Metamorph and for handling large data sets which arise from experiments that employ Andor cameras.

Features:

High end Windows^® workstation
Acquisition bandwidth for dual camera support
ImageDisk removes dependency on RAM for huge datasets
Multi-core processors for multi-threaded software
High performance graphics with OpenGL
Real-time volume rendering with Bitplane Imaris option
Designed to run MetaMorph for Olympus Premier configured for the Andor XD

Motorized XY & Z Control

Automation


Z Stage Features Piezo stages with 100, 200 and 500 μm ranges 1 nm repeatability 2-8 nm resolution (depends on range) Up to 100 Z sections/s Specimen holders - slides, petri and microtitre plates	XY Stage Features Open and closed loop stages Typical travel >100 x 75 mm, with 0.02 μm resolution 30 mm/sec travel speed Perform multi-field scans for 6D imaging Create 4D mosaics Repeatability 0.2 to 0.3 μm rms	Piezo Objective Features PI PIFOC P721 - 100 μm travel PI PIFOC P725 - 400 μm travel Up to 20 Z sections/s 1.25 nm resolution Analog or digital control Oil and water objectives

Camera Port Adaptors

Single & Dual Port

Single Port Coupler Features

Magnification X = 1.0, 1.2, 1.5, 2.0 (others by request)
Compatible with filter wheel or CSU+ filter wheel
Achromatic 450-650 nm
AR-coated
Transmission > 98%
Adjustable XY centering
Sliding barrel focus control

Dual Port Coupler Features

Enables simultaneous two-camera exposure with discrimination by wave length or polarization. A 100% mirror can be included for easy camera switching.

Magnification X = 1.0, 1.2, 1.5, 2.0 (others by request)

Compatible with 'C'/'CS' lenses, C-mount, filter wheel or CSU+filter wheel

Magnification select in each arm

Mounting cassettes 'store adjustment' simplifying exchange and minimizing readjustment

Filter Wheels & Splitters

Emission discrimination

TR-FW10 Features

6,10 & 12 position, ø25 filter wheels
Optional support for Ludl, Prior, ASI and Yokogawa wheels
Matched to CSU or microscope C-mount coupler
Infinity optical path for optimum filter performance
External controller allows support of Sutter SmartShutter

Field Splitter Features

Optosplit II and III which use 25 mm filters
Field splitting for simultaneous 2 or 3 color imaging
Matched to CSU or microscope C-mount coupler
DualViewTM and QuadView also supported

MetaMorph is a registered trademark of MDS Analytical Technologies, Inc.
Andor, the Andor logo and Revolution are trademarks of Andor Technology Plc.

Laser