Scaleview

Tissue clearing with the SCALEVIEW-A2 solution has opened up new possibilities for deep imaging. When coupled with SCALEVIEW-A2 clearing, Olympus SCALEVIEW immersion objectives now allow researchers to reconstruct vast tissue areas at high resolution with the FV1200MPE system.
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SCALEVIEW Immersion 25X Objectives for Multiphoton A2

The Power to Visualize 3-Dimensional Structures at Unprecedented Depths in Morphologically Intact Tissue

For more than a century, researchers have had to section tissue in order to visualize large tissue areas, disrupting tissue and data in the process. Tissue clearing with the SCALEVIEW-A2 solution has opened up new possibilities for deep imaging. When coupled with Olympus SCALEVIEW immersion objectives, researchers can now reconstruct vast tissue areas at high resolution with the FV1200MPE system.

Scaleview A-2
SCALEVIEW-A2 Optical Clearing Agent

SCALEVIEW-A2 revolutionizes the imaging of formalin-fixed specimens.

  • Simply render mammalian brain tissue transparent through immersion in SCALEVIEW-A2 solution.
  • SCALEVIEW-A2 eliminates light scattering, allowing clear imaging of fluorescently labeled structures in detail from the surface to significant depth.
  • Eliminates the need for mechanical sectioning
  • Does not degrade fluorescence like BABB and similar techniques
SCALEVIEW-A2 contains the fundamental components of ScaleA2 developed by the RIKEN Brain Science Institute in Japan. It is adjusted to achieve the optimal performance with the SCALEVIEW objectives. Read the RIKEN article in Nature Neuroscience

SCALEVIEW-A2 specifications

Item Specifications
Optical characteristic Refractive index ne (23 °C) 1.380
Abbe number ve (23 °C) 51
Volume 1000 ml
Storage temperature 2 - 35 °C
  • BX3 fits meDP80
  • special promoLV200
  • VS120 Slide Systemfvmpe_rs
  • Specialty Objectives Imaris by Bitplane
  • CX22cellsens Digital Imaging Software