CV1000  Spinning Disk Confocal

The CellVoyager™ CV1000 provides renowned Yokogawa spinning disk confocal imaging technology in an easy to use, incubated bench-top solution for live cell imaging. With its microlens enhanced dual Nipkow disk scanning technology, phototoxicity and photobleaching are drastically reduced, making it ideal for use in observing highly delicate life processes such as iPS/ES cell generation and embryogenesis. From multiple 35mm dishes to multi-well plates, the CV1000 has the throughput to handle some of your most demanding time-lapse research.

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DATA Courtesy of Kazuo Yamagata, PhD, Wakayama Lab.(Laboratory for Genomic Reprogramming),Center for Developmental Biology, RIKEN

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Following the injection of mouse embryos with mRNA, nearly 25,000 multicolor and multilayer confocal images of the embryos were acquired over 60 hour period as they developed to the blastocysts stage.Thereafter, they were transferred to a recipient mouse that gave birth to healthy pups, each of which developed normally and had full reproductive capability. This is firm evidence that long-term, multi-dimensional confocal imaging with CV1000 causes no harm to a delicate specimen such as an early stage mouse embryo.

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Wide-area imaging of tissue sections

Interkinetic nuclear movement in the cerebral cortex of chicken
Total Time 20 hours
Interval 10 min
Z-section/stack 25 sections (2µm / apart)
Imaging positions 1 field
Total images 6,000 images
(6,000 images/field)
Excitation wave length 488nm Neuron: GFP
561nm Nucleus: mCherry
Objective lens 20x Dry

Interkinetic nuclear movement in the Cerebral cortex of chicken was observed for about 20 hours. Confocality and high-precision motorized stage movement of the CV1000, makes it possible to observe biological reactions in thick specimens.

Data Courtesy of Yuji Watanabe, PhD, Tohoku University, Development of Molecular Neurobiology, Graduate school of Life Sciences

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In vivo imaging of white blood cells patrolling zebra fish

Total Time 3 hours
Interval 1 min
Z-section/stack 20 sections (3.2µm / apart)
Imaging positions 1 field
Total images 7,200 images
(7,200 images/field)
Excitation wave length 488nm Neuron: EGFP
561nm Cytoplasm: mKate2
Objective lens 20x Oil

Multi-color, high resolution data of 3D movement in live specimens can be acquired by rapidly capturing Z-slice images.

Data Courtesy of Philipp Niethammer, Harvard Medical School Mitchison Lab

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96-well plate imaging

Image shown is compilation of sequentially captured data using multiple parameters. 60 wells can be imaged in 2.3 minutes.

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Plant Imaging

Imaging of the vacuolar membrane shape during germination process in arabidopsis thaliana
Total Time 14 hours
Interval 1 min
Z-section/stack 11 sections (1µm / apart)
Imaging positions 6 fields
Total images 55,440 images
(9,240 images/field)
Excitation wave length 488nm Vacuolar membrane: Vam3-GFP
Objective lens 60x Oil

Changes in the shape of a vacuolar membrane during the germination process were continuously recorded. High-speed and multi-point time lapse imaging with the CV1000 allows accurate and high-resolution tracking of rapid changes in living organisms, something that has proven quite difficult with conventional imaging systems.

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GFP transfected 293F cells

Total Time 20 hours
Interval 10 min
Z-section/stack 101 sections (0.3µm / apart)
Imaging positions 25 fields
Excitation wave length 488nm Neuron
Objective lens 60x Oil

Floating 293F cells were transfected with eGFP by using NeoFection. The cells were shake-cultured overnight. Using time-lapse imaging, active movement of cells expressing eGFP and structual changes in the cell wall were clearly observed inside the floating cell clusters.

Data Courtesy of Astec. Co., Ltd.

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